Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • oiiio
    Senior Member
    • Jan 2011
    • 105

    #1

    Why don't mapping programs map directly into BAM format?

    Is this historical contingency? Or is there a functional reason why mapping programs like BWA or Stampy don't map directly to BAM?
  • lh3
    Senior Member
    • Feb 2008
    • 686

    #2
    1) because writing BAM is non-trivial and no one wants to add a dependency only for writing BAM

    2) because you can easily generate BAM by piping the output to samtools

    Comment

    • adaptivegenome
      Super Moderator
      • Nov 2009
      • 436

      #3
      But assuming it was trivial, is there a good reason to output to SAM. Seems like all downstream operations would be BAM-based anyways?

      Comment

      • simonandrews
        Simon Andrews
        • May 2009
        • 870

        #4
        When mapping sequences each sequence is handled independently and the mapping program just wants to write this to the output file and move on (even more so when running multiple threads).

        Many people who want to use BAM files want to have indexed files which allow for efficient random access. Because the files need to be sorted the output needs to be post-processed after all of the mapping is done, you can't just write them out as you go along. Mapping programs would therefore still have to write out SAM files as they went along and then convert these to BAM at the end. Since you can easily do this with a samtools command the mapping programs generally don't bother to do this and leave it up to the user.

        Comment

        • maubp
          Peter (Biopython etc)
          • Jul 2009
          • 1544

          #5
          Indeed. Or to put it another way,

          Output of unsorted SAM is easy.

          Output of pre-sorted SAM/BAM is hard.

          Since you (or the tool) will have to do a SAM/BAM coordinate sorting step anyway, you might as well do the unsorted SAM to sorted BAM in one go with samtools.

          Comment

          Latest Articles

          Collapse

          • SEQadmin2
            Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
            by SEQadmin2



            Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

            Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
            ...
            10-14-2026, 09:55 AM
          • SEQadmin2
            New Genomics Technologies Take Aim at Long-Standing Limits
            by SEQadmin2


            Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

            We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
            ...
            09-28-2026, 10:25 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by SEQadmin2, Yesterday, 11:13 AM
          0 responses
          19 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, Yesterday, 10:48 AM
          0 responses
          18 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-15-2026, 10:52 AM
          0 responses
          36 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-14-2026, 05:39 AM
          0 responses
          42 views
          0 reactions
          Last Post SEQadmin2  
          Working...