Hi,
I have aligned paired-end read data from from a gene resequencing project, and am attempting to run the CalculateHsMetrics tool from PicardTools suite to calculate various metrics, and have a question re interval lists.
In order to derive % on-target, I need to define locations of baits, and target intervals. This is not a problem, but I cant figure out what the header should look like. The main reference often referred to re formatting of the interval list header is this: http://picard.sourceforge.net/javado...ervalList.html
... but I was looking for an actual example, and/or how to generate a header that will work with my processed bam file.
Thanks in advance,
Dave
I have aligned paired-end read data from from a gene resequencing project, and am attempting to run the CalculateHsMetrics tool from PicardTools suite to calculate various metrics, and have a question re interval lists.
In order to derive % on-target, I need to define locations of baits, and target intervals. This is not a problem, but I cant figure out what the header should look like. The main reference often referred to re formatting of the interval list header is this: http://picard.sourceforge.net/javado...ervalList.html
... but I was looking for an actual example, and/or how to generate a header that will work with my processed bam file.
Thanks in advance,
Dave
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