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  • Cirno
    Junior Member
    • Jun 2011
    • 6

    #1

    [Mosaik] - parameter settings

    Hello!

    I am using Mosaik Aligner on 2x100bp paired end reads;
    -mm <mismatches> the # of mismatches allowed
    Does that mean mm in each pair member individually (assuming this), or the entire stretch? Any suggestions/wisdom on any other settings that people have out there would also be much appreciated!

    Secondly, I have a question about the SAM output it creates:
    Usually, in tophat, I could expect some of the following information at the end of each line in the SAM file:
    NM:i:1 XS:A:+ NH:i:1
    I am now seeing things like..
    G:Z:ZGL39GTJ7H8 NM:i:9 MD:Z:84^T1G1T0T1T0T1T0G1T
    Anyone know what those would all be about?

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