Here are some terms definition from samtools manual.
Then
My question is what is next segment? My major is not bioinformatics, so sorry for the silly question.
Thanks a lot.
Code:
Template: A DNA/RNA sequence part of which is sequenced on a sequencing machine or assembled from raw sequences. Segment: A contiguous (sub)sequence on a template which is sequenced or assembled. For sequencing data, segments are indexed by the order in which they are sequenced. For segments of an assembled sequence, they are indexed by the order of the leftmost coordinate on the assembled sequence.
Code:
RNEXT: Reference sequence name of the NEXT segment in the template. For the last segment, the next segment is the rst segment in the template.
Thanks a lot.
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