I used tophat to map paired end seq data xxx.1.fq and xxx.2.fq.
from the output file, I found most of reads are paired. the flags usually are
163 - 83
355 - 403
99 - 147
....
my question is:
how to determine the pair is from "+" strand or "-" strand?
thanks
from the output file, I found most of reads are paired. the flags usually are
163 - 83
355 - 403
99 - 147
....
my question is:
how to determine the pair is from "+" strand or "-" strand?
thanks
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