how to identify the ideal -c "minimum number of alignments in a locus for needed to conduct significance testing" (--min-alignment-count) to be used in cuffdiff?
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I want to find out too, the default is 1000. How do we set the best value for different RNA-Seq data? Anyone can answer this? -
The default, when using Galaxy, is set to 1000. However, the documentation says this value is set to 10 by default. I'm curious as to this discrepancy and the best value for RNA Seq experiments.
-c/--min-alignment-count <int> The minimum number of alignments in a locus for needed to conduct significance testing on changes in that locus observed between samples. If no testing is performed, changes in the locus are deemed not signficant, and the locus' observed changes don't contribute to correction for multiple testing. The default is 10 fragment alignments.Comment
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Hi, yes, I noticed that too... was it a mistake on the documentation or on Galaxy? but I had set 100 on my data.Comment
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Galaxy just changed it to 10, seems a mistake.Comment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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