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  • songsy
    Junior Member
    • Oct 2011
    • 6

    #1

    RDXplorer

    I'm using SVmerge. One of the tools is RDXplorer. I run the run.sh. It first picked q0 items and write into a sam file. And then it uses samtools mpileup to call variants. But it doesn't work, showing that :
    [bam_header_read] EOF marker is absent. The input is probably truncated.
    [bam_header_read] invalid BAM binary header (this is not a BAM file).

    The script truncate the bam file itself and it doesn't move on. What can I do?
    Thank you very much
  • gprakhar
    Member
    • Aug 2010
    • 78

    #2
    Hello,

    Something wrong with your bam!
    BTW Is it a bam?

    --
    pg

    Comment

    • eulbra
      Junior Member
      • Mar 2012
      • 8

      #3
      I add a com in

      if fu.isExist(q_mapped_sam):
      com = 'wc -l ' + q_mapped_sam + ' > ' + q_mapped_sam + '.log'
      v6.execute(com, debug)
      com = glob.SAMTOOLS_PATH + '/samtools view -T ' + reference +' -bS '+ q_mapped_sam + ' > ' + q_mapped_bam
      v6.execute(com, debug)
      com = glob.SAMTOOLS_PATH + '/samtools mpileup -Sf ' + reference + ' ' + q_mapped_bam + ' > ' + q_pileup
      v6.execute(com, debug)

      then it work, but I still can not run through the whole programe.

      Originally posted by songsy View Post
      I'm using SVmerge. One of the tools is RDXplorer. I run the run.sh. It first picked q0 items and write into a sam file. And then it uses samtools mpileup to call variants. But it doesn't work, showing that :
      [bam_header_read] EOF marker is absent. The input is probably truncated.
      [bam_header_read] invalid BAM binary header (this is not a BAM file).

      The script truncate the bam file itself and it doesn't move on. What can I do?
      Thank you very much

      Comment

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