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  • swebb
    Junior Member
    • Jun 2009
    • 7

    Coverage plots for paired end data

    We have been creating coverage plots from chip-seq bam files using tools such as genomeCoverageBed or bamToBigWig. With single end chip-seq data we have not been extending read length to an inferred fragment size. However with paired end data it seems to make sense to do this if you have good quality pairs.

    Are there any coverage plotting tools that will do this automatically? I.e look at aligned pairs and output coverage including the region between each.

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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM
  • SEQadmin2
    From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
    by SEQadmin2


    Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
    ...
    06-02-2026, 10:05 AM

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