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Does anyone know of a script that allows one to convert Qual scores from a fastq file in order to map w/ BWA or Bowtie using the command line? I'm familar with the Galaxy tools function, but Galaxy has really been unreliable lately, I'd love to move away from it whenever possible.
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I may be confused by your question, but BWA/bowtie take fastq files as input. -
The qual portion of fastq comes in different varieties. Bwa and bowtie only accept one format. The galaxy fastq groomer changes the qual score format to allow bwa or bowtie compatibility. I would like to run that script from the command line, and I wondered if anyone knew where to find it.Comment
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Not true. Both bwa and bowtie can use directly FASTQ files with Q-scores encoded as either Phred+33 (Sanger standard) or Phred+64 (Illumina prior to CASAVA 1.8). You just need to set the proper option on the command line.Originally posted by Giles View Post
Check the manuals for both of these programs for specifics.Code:bowtie: --phred-33 (default) or --phred-64 bwa aln: (default is Phred+33, no option required) or -I (Phred+64).
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There are several other options for doing the conversion if you need to, e.g. EMBOSS seqret
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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