I have got 75bp single end RNA-seq data from 8 samples. And I would like to pool reads from multiple samples to run Tophat. The problem is, we will be running cufflinks pipeline after mapping. And cuffdiff requires bam files per sample. Is there any way I can split the bam file after Tophat run? How can I give separate Readgroup tags to each sample in my Tophat run?
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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