Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • empyrean
    Member
    • Sep 2010
    • 52

    Cut the reads.. paired end fastq file

    hi.. i have the following 150bp reads.. i would like to cut the bases which are more than 100bp. Also i would like to cut from begining of the read. please let me know any script or program which can do this.

    Code:
    @HWI-DO4456:7:000000000-Z07CL:1:1:15052:1479 1:N:0:GGCTAC
    TCCTCAGATTTTTTAGAAAGAGGAGTCTGCTTATAAGATAATGGCATCATTTTGATAGAATCTCCTCGCATTGTTGTAAAACTAATAACAAAGAAGGTTGGTTTTTGTGGTTTTGGTCTCCCGGCCTGAATCCAAGCTTGATGAATACGAA
    +
    @CCFFFFFHHHHHJJIJJJJJJIJJJJJJJJJIJJJIJJJIJJJJJJJJJJJJIJJJJJJIJGJJJJJJFHHFFFFFEEEEDEDDDDDDDCDDCBDD>@CB?BDDDDDDDCBDDDBBCDDDDDDDBDDDDDDDDDCBCDCBCDDDDEDDDD
    @HWI-D04456:7:000000000-Z07CL:1:1:17590:1511 1:N:0:GGCTAC
    TTAATTATACTTGTTGGTTTTGGTGGCGGATTAACATGGGGAGCAGTCGCTCTTCGTTGGGGTAAATAAGGACTGAGAGAAAAAAAGGAGTGTATTTTGTGAAGGTAGGGGCACAGTACCGTTGAAGCGTCTAATGAACGTGGAGGGATGG
    +
  • maubp
    Peter (Biopython etc)
    • Jul 2009
    • 1544

    #2
    Do you mean keep the left end, or keep the right end?

    What is your favourite scripting language (since there are lots of options here, Perl, Python, Ruby, etc)?

    Comment

    • empyrean
      Member
      • Sep 2010
      • 52

      #3
      i use perl.. keep the right end.. and trim the left end.. i already trimmed adapters..

      Comment

      • dgtnk
        Junior Member
        • Nov 2011
        • 4

        #4
        perl is OK
        fastx_trimmer provided by FASTX_Toolkit may be faster.

        Comment

        • RogerH
          Junior Member
          • Dec 2011
          • 6

          #5
          I recently used fastx_trimmer on my data and I can only recommend it.

          Comment

          • solaymane
            Junior Member
            • Jun 2012
            • 2

            #6
            hello i am new member beginner to the field

            Comment

            Latest Articles

            Collapse

            • SEQadmin2
              Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
              by SEQadmin2



              Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
              ...
              07-09-2026, 11:10 AM
            • SEQadmin2
              Cancer Drug Resistance: The Lingering Barrier to Rising Survival
              by SEQadmin2



              Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

              There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
              07-08-2026, 05:17 AM
            • GATTACAT
              Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
              by GATTACAT
              Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
              07-01-2026, 11:43 AM

            ad_right_rmr

            Collapse

            News

            Collapse

            Topics Statistics Last Post
            Started by SEQadmin2, Today, 10:26 AM
            0 responses
            9 views
            0 reactions
            Last Post SEQadmin2  
            Started by SEQadmin2, 07-09-2026, 10:04 AM
            0 responses
            24 views
            0 reactions
            Last Post SEQadmin2  
            Started by SEQadmin2, 07-08-2026, 10:08 AM
            0 responses
            16 views
            0 reactions
            Last Post SEQadmin2  
            Started by SEQadmin2, 07-07-2026, 11:05 AM
            0 responses
            33 views
            0 reactions
            Last Post SEQadmin2  
            Working...