DEseq: zero differentially expressed regions found

[rebrendi]

Hello,

I am running DEseq for the first time.
With the default parameters, it returned zero differentially expressed regions.
Are there any known caveats?

Thanks!

[rebrendi]

solved, sorry

[aggp11]

could you post what the reason was for observing zero differentially expressed genes?

Thanks

[rebrendi]

I still don't know, I just used the Audic-Claverie algorithm instead, so for the time being I hope that will be enough for my task.

The parameters that I used for DESeq were as follows:
p-value threshold =0.01
log2 fold change is ≥ 1
the two samples tested were without replicates.