Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • phatjoe
    Member
    • Aug 2011
    • 13

    #1

    Clarification on BCF file format

    I have a bcf file with this format.

    HTML Code:
    ChrM   21  .   A   T,G,X   0   .   DP=3796;I16=3730,0,55,0,135424,4994460,1972,72624,42241,1163355,634,7544,26664,192714,488,4340  PL  0,255,199,255,217,199,255,217,199,199
    DP should be the depth for the base called I pressumed. But what does the I16 field which is succeeded with 16 other comma delimited numbers mean?

    Also as for PL,these numbers 0,255,199,255,217,199,255,217,199,199 are the likelihood of the alleles to be P(D|AA),P(D|AT),P(D|AG),P(D|AX),P(D|TT),P(D|TG),p(D|TX),p(D|GG),p(D|GX) and p(D|XX) in chronological order,right?

    Please enlighten me. I cant seemed to find the link to any page describing the bcf output!

    Thanks in advance!
  • jflowers
    Member
    • Oct 2011
    • 42

    #2
    Check the documentation under the section "SAMtools/BCFtools specific information" here: http://samtools.sourceforge.net/mpileup.shtml

    Comment

    • phatjoe
      Member
      • Aug 2011
      • 13

      #3
      Originally posted by jflowers View Post
      Check the documentation under the section "SAMtools/BCFtools specific information" here: http://samtools.sourceforge.net/mpileup.shtml
      Overlooked it. Thanks a bunch!

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM
      • SEQadmin2
        New Genomics Technologies Take Aim at Long-Standing Limits
        by SEQadmin2


        Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

        We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
        ...
        09-28-2026, 10:25 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 10-22-2026, 11:13 AM
      0 responses
      26 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 10:48 AM
      0 responses
      20 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-15-2026, 10:52 AM
      0 responses
      36 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-14-2026, 05:39 AM
      0 responses
      43 views
      0 reactions
      Last Post SEQadmin2  
      Working...