Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • astat
    Junior Member
    • Jan 2012
    • 3

    #1

    Genome alignments

    Hello! My question is on how to make a "fake" reference genome from a set of contigs. I have the following data available:

    -Assembled contigs from my species (from genomic data, but I only have the contigs)
    -A reference genome (different species)
    -Linkage maps for both

    I would like to make an alignment between the contigs from my species and the reference, then concatenate the contigs from my species into a fake genome based on the order in the reference and/or linkage maps. The contigs are long (several kb), and I expect there to be many gaps due to introns, repeats, etc.

    Any suggestions for where to start? Thanks!
  • maasha
    Senior Member
    • Apr 2009
    • 153

    #2
    Take a look at Mummer for making the alignments.

    Comment

    • lh3
      Senior Member
      • Feb 2008
      • 686

      #3
      For mammalian genomes, bwa-sw.

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM
      • SEQadmin2
        New Genomics Technologies Take Aim at Long-Standing Limits
        by SEQadmin2


        Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

        We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
        ...
        09-28-2026, 10:25 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 10-22-2026, 11:13 AM
      0 responses
      27 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 10:48 AM
      0 responses
      20 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-15-2026, 10:52 AM
      0 responses
      36 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-14-2026, 05:39 AM
      0 responses
      43 views
      0 reactions
      Last Post SEQadmin2  
      Working...