Problems with running Quake

[vivienne_lovely]

Hi, I am new to this area and I am currently working on Highseq data analysis.There are some problems with running quake. I have this output from the software Quake:

terminate called after throwing an instance of 'mapped_file::ErrorMMap'
what(): Can't open file '/Quake/bin/test/a.dbm':
/bin/sh: line 1: 29698 Aborted /Quake/bin/jellyfish qdump -c /Quake/bin/test/a.dbm > a.list.qcts
Optimization of distribution likelihood function to choose k-mer cutoff failed. Very likely you have set the value of k too high or not provided adequate coverage (>15x). Inspect the k-mer counts for a clear separation of the error and true k-mer distributions.
Guessing quality values are on ascii 64 scale
Traceback (most recent call last):
File "./quake.py", line 287, in ?
main()
File "./quake.py", line 124, in main
cutoff = get_cutoff()
File "./quake.py", line 163, in get_cutoff
exit(1)
TypeError: 'str' object is not callable

I changed the value of K,but the problem still there. I do not know how to solve this problem.
Thanks for anyone that could help.

[dcopetti]

I had several different problems using Quake, and when I got your error it was due to a wrong guessing of the ascii scale. Try using -q parameter (it should be in 33 scale in your case) when you use quake.py, or count-qmers and correct commands.
I hope this helps!