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  • jpofmars
    Junior Member
    • May 2011
    • 4

    #1

    Kmer overrepressented in an exome analysis

    Hi,

    I have a problem with an exome analysis. The sample was sequenced with illumina HiSeq 2000 in paired-end with 100 cycles.
    When I run FASTQC to see the quality of my samples, I have kmers which are overrepresented. (TTTTT, AAAAA, GGCGG). I join the picture of kmer content.

    I have poor feedback on this kind of analysis, whereas it is the first sample (for exome) where I see that.

    Do you think there was a problem during the capture of exome ? Or is it a specification of my sample ?

    Thanks.
    Attached Files
  • jpofmars
    Junior Member
    • May 2011
    • 4

    #2
    For information, I perform the alignment and I see if all the exoms were covered with bedtools.
    The bedfile of the exom was genereted with ucsc.

    On 389394 exoms 244372 have a coverage of 0.

    Comment

    • amitm
      Member
      • Feb 2011
      • 52

      #3
      Trim bases from 5'-end

      hi jpofmars,
      Though I haven't done an Exome analysis, but I have seen the same issue with RNA-Seq data. As per the comment posted by BabrahamBioinf, creator of FastQC (@ YouTube page of FastQC tutorial vid -> link)

      The random hexamers used during priming the library are not "that random" and hence u see the high peaks of k-mer at the 5' end for initial 10 bases or so. At the 3' end we use oligo-dT.

      In my experience (RNA-Seq), trimming the first 3 bases from 5' end and trimming a couple of bad quality bases from the 3' end, majorly reduces the k-mer occurrence.

      Attached are k-mer profile plots before and after trimming.
      Attached Files

      Comment

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