Hi can anyone say me how i can find perfect matches and mismatches present in a probe i mean i want to find probes having 1 mismatch, probes having 2 mismatches..............
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i have probe information for hg18 genome i need to find pm and mm for them with mcf7 cancer cell line (for the reads mapped to the probes whether probe location is perfectly matched with them or how many mismatches are present for every probe)Last edited by bhargavpbk88; 01-19-2012, 09:32 AM.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...-
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