Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • yaaraore
    Junior Member
    • Oct 2011
    • 3

    #1

    problems with zero counts using cufflinks

    I encountered a problem during my last run of cufflinks. From some resons I got that when sample1 is zero and the other is a number (even a very high one like 7000) it returns that the test was not significant. I ran a different genome with the same parameters and this time I did not get this problem (I ran versions 1.3.0 and 1.2.1).
    Any idea why something like this happens?
  • jmwhitha
    Senior Member
    • Mar 2013
    • 107

    #2
    Did you ever figure this out? I am also having this problem. My p-value and q-values are defaulting to 1 while my test_stat is defaulting to zero for value_1 or value_2 = 0 or close to zero compared to very high values.

    In addition to this I am getting LOWDATA status despite clearly very high expression (160k count vs 850 count, for example).

    Any help would be much appreciated!
    Jason

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, Yesterday, 11:13 AM
    0 responses
    16 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, Yesterday, 10:48 AM
    0 responses
    17 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    35 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    42 views
    0 reactions
    Last Post SEQadmin2  
    Working...