I have a control and a knockdown experiment from which I am sequencing small rnas. When I count the sum of the reads mapped to the known mirnas foreach condition I get 968384 for contol anD 71736 for the knockdown. I really dont know how to interpret the results and if normalizing by the total nr of reads makes sence. Important I cannot use the assumption that the expression of most miRNAs should not change. Any suggestions?
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...