Protein data base with blast+

[detq182]

Hi everyone.

im new in this forum and my problem is that i download a database from ncbi with tar.gz format them extract it on /db/ and use

blastx -query Lib1_consensus_tgicl_NT_nohit.fasta -db swissprot.00 -out one.txt

i think that the problem its the db cause with others db work this comand

Note: i also use -db swissprot but same results. by the way the db that i download was swissprot.tar.gz

thanx

[cliffbeall]

You should give the error message cause it could be a lot of things.

That said it sounds like a path/environment issue. You may either need to specify a path to the db (something like -db /db/swissprot), or modify your environment so it knows where to look.
More info here (check the configuration section): http://www.ncbi.nlm.nih.gov/staff/ta...nix_setup.html

[GenoMax]

This may be a long shot but what version of windows are you using to do this? Is it 32-bit or 64-bit?