When annotating a eukaryotic genome de novo, I find it tricky to identify gene models that in reality are two genes that are incorrectly fused. Rather than correctly identifying the stop codon, the gene finder adds an intron and continues the gene model into what really is a neighboring gene. I was just wondering what you do to identify these incorrectly fused gene models. Blasting all gene models is probably the obvious start, but then how to fish out the incorrectly fused ones and visualize the results? Are there any available tools that does this?
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM -
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM
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