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How to assemble reads in FASTA format by Celera Assembler? What are the minimum input files to start assembling in Celera.
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There is a lot of documentation on the project website. There is also some helpful information on the UMD website. The Celera Assembler is really a pipeline of programs and you will want to pay special attention to the way your data (long reads vs. short reads) needs to be processed with this assembler.Originally posted by shuang View Post
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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