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  • coverage analysis

    I have to analyze small RNA sequence reads from illumina platform. (40bp reads,illumina1.5:quality encoding).
    reference genome is annotated and i am interested in determing the coverage of my mapped reads to reference genome features.
    i have mapped the reads to reference genome by TopHat and Bowtie generating .bam files
    i then sorted and indexed my .bam files

    now i want to study coverge of each annotated feature of the reference genome.
    How shall i go about it?

  • #2
    bedtools coverageBed?

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    • #3
      GATK DepthOfCoverage is nice.

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