Hi all i m shahid. I want to sequence Pollen beetle (Meligeteus aeneus) genome using paired-end technique with Illumina system. Can any body help me to find reference sequence for this pollen beetle genome alignment.
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Hey Shahid,Originally posted by shahid.manzoor View Post
First off I'd put this question in a new thread under an appropriate title. I don't know if you reaized but you put your comment at the end of an existing thread on a different topic.
If I were you I'd look at the NCBI Taxonomy browser to try to find closely related species with completed genomes. http://www.ncbi.nlm.nih.gov/Taxonomy/
I have heard it said that you need > 95% sequence similarity for resequencing to work, but I'm sure that this boundary will be pushed back with algorithmic enhancements (moving from re-sequenceing approach towards a de-novo sequencing approach).
Good luck with your work!
Dan.
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