Hi all i m shahid. I want to sequence Pollen beetle (Meligeteus aeneus) genome using paired-end technique with Illumina system. Can any body help me to find reference sequence for this pollen beetle genome alignment.
-
-
Hey Shahid,Originally posted by shahid.manzoor View Post
First off I'd put this question in a new thread under an appropriate title. I don't know if you reaized but you put your comment at the end of an existing thread on a different topic.
If I were you I'd look at the NCBI Taxonomy browser to try to find closely related species with completed genomes. http://www.ncbi.nlm.nih.gov/Taxonomy/
I have heard it said that you need > 95% sequence similarity for resequencing to work, but I'm sure that this boundary will be pushed back with algorithmic enhancements (moving from re-sequenceing approach towards a de-novo sequencing approach).
Good luck with your work!
Dan.
-
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment