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  • shuang
    Senior Member
    • Jul 2011
    • 100

    #1

    errors with PacBioToCA in Celera

    I'm learning to use Celera to assemble PacBio sequences. However, I can NOT make pacBioToCA working.

    I'm stock with pacBioToCA command when I'm following the example exercise on http://sourceforge.net/apps/mediawik...tle=PacBioToCA

    ./fastqToCA -libraryname illumina -technology illumina -type sanger -innie -reads illumina.fastq > illumina.frg

    java convertFastaAndQualToFastq wgs-7.0/Linux-amd64/bin/sampleData/pacbio.filtered_subreads.fasta > wgs-7.0/Linux-amd64/bin/sampleData/pacbio.filtered_subreads.fastq

    ./pacBioToCA -length 500 -partitions 200 -l pacbio -t 16 -s pacbio.spec -fastq pacbio.filtered_subreads.fastq illumina.frg


    Then, here came an error message:

    Running with 16 threads and 200 partitions
    AMOS binaries: bank-transact not found in
  • fturner
    Junior Member
    • Apr 2011
    • 1

    #2
    have you installed AMOS libaries (see other requirements at http://sourceforge.net/apps/mediawik...ng_from_Source ) ? This error went away when I did this and added the directory src/Bank in the the AMOs directory to my path.

    Comment

    • shuang
      Senior Member
      • Jul 2011
      • 100

      #3
      After I set the path pointing to amos directory, the problem was solved. Thanks!

      Comment

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