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  • adaptivegenome
    replied
    Realignment is performed on a set of reads. If you merge two BAMs and add reads to a locus then realignment is needed. Same goes for marking duplicates or recalibrating bases.
    Last edited by adaptivegenome; 02-17-2012, 04:39 PM. Reason: typo

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  • memento
    started a topic Merging bam files

    Merging bam files

    According to best practice variant detection if You have multiple sequencing of the same sample from 2 different runs You need to merge bam files prior to realigner/indel realigner/mark duplicates. Is it possible to merge bam files that were already realigned/marked duplicated (per each bam separately) ?
    Last edited by memento; 02-17-2012, 12:21 PM.

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