I assigned RGID's to 4 different sets of files. I labeled them L1, L2, L3, L4 to indicate which lane they were from. I was having issues after samtools merge and I think the problem is that the first in.bam header overwrote the other headers (this is in the samtools manual). Basically I have orphan reads now because they have a RGID that isn't in the header. All the other RG tags are identical in the bam files. I was told that I needed to add the other ID's to my header in the merged file. So I guess the header needs to say something like RGID: L1, L2, L3, L4 for it to work properly but I don't think you can put comma's. I don't really know how to reformat text files and on a scale of 1-10 in programming knowledge, I would put myself at a 2. Can someone please give me an example code for how to edit this header? I will attach the header of my existing file below if that helps.
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I think various RGs need to be on separate lines.
You can use text editor to get the header you want, no "programming" involved using gedit/vim/emacs.
It you did want to program, using the Unix tools sed,grep,cat and so in a script could solve the problem.
When you get the header you want, try the samtools "reheader" option.
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Originally posted by Richard Finney View PostI think various RGs need to be on separate lines.
You can use text editor to get the header you want, no "programming" involved using gedit/vim/emacs.
It you did want to program, using the Unix tools sed,grep,cat and so in a script could solve the problem.
When you get the header you want, try the samtools "reheader" option.
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Originally posted by shawpa View PostI think I wasn't clear when I said I couldn't program very well. I actually meant I couldn't program as well as do what you are describing.
Right now your header has only L1:
Code:@RG ID:L1 PL:ILLUMINA PU:D0DHVACXX LB:ryan SM:ryan
Code:@RG ID:L1 PL:ILLUMINA PU:D0DHVACXX LB:ryan SM:ryan1 @RG ID:L2 PL:ILLUMINA PU:D0DHVACXX LB:ryan SM:ryan2 @RG ID:L3 PL:ILLUMINA PU:D0DHVACXX LB:ryan SM:ryan3 @RG ID:L4 PL:ILLUMINA PU:D0DHVACXX LB:ryan SM:ryan4
The method Richard Finney suggests is to use samtools reheader:
Code:samtools view -H file.bam > header.txt ...edit header.txt using any text editor... samtools reheader header.txt file.bam > file.fixedheader.bam
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