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  • c_ro87
    Member
    • Feb 2012
    • 12

    #1

    get mate pair info from xml file

    Hello i'm trying to assembly a sequencing done 100% with sanger reads, using celera assembler

    i have the fasta, fasta.qual, anc, clip and xml files from NCBI Trace

    the xml files have the mate pair info, but when i run the assembler, skip this info

    from the final .qc file

    [Mates]
    ReadsWithNoMate=691348(100.00%)
    ReadsWithGoodMate=0(0.00%)
    ReadsWithBadShortMate=0(0.00%)
    ReadsWithBadLongMate=0(0.00%)
    ReadsWithSameOrientMate=0(0.00%)
    ReadsWithOuttieMate=0(0.00%)
    ReadsWithBothChaffMate=0(0.00%)
    ReadsWithChaffMate=0(0.00%)
    ReadsWithBothDegenMate=0(0.00%)
    ReadsWithDegenMate=0(0.00%)
    ReadsWithBothSurrMate=0(0.00%)
    ReadsWithSurrogateMate=0(0.00%)
    ReadsWithDiffScafMate=0(0.00%)
    ReadsWithUnassignedMate=0(0.00%)
    TotalScaffoldLinks=0
    MeanScaffoldLinkWeight=0.00



    the xml file looks like


    <trace>
    <CENTER_NAME>JGI</CENTER_NAME>
    <CLIP_LEFT>0</CLIP_LEFT>
    <CLIP_RIGHT>690</CLIP_RIGHT>
    <SOURCE_TYPE>GENOMIC</SOURCE_TYPE>
    <SPECIES_CODE>XX</SPECIES_CODE>
    <SUBMISSION_TYPE>NEW</SUBMISSION_TYPE>
    <TEMPLATE_ID>PACH2306</TEMPLATE_ID>
    <TI>451441523</TI>
    <TRACE_DIRECTION>FORWARD</TRACE_DIRECTION>
    <TRACE_FORMAT>SCF</TRACE_FORMAT>
    <TRACE_NAME>PACH2306.x1</TRACE_NAME>
    <TRACE_TYPE_CODE>WGS</TRACE_TYPE_CODE>
    </trace>
    <trace>
    <CENTER_NAME>JGI</CENTER_NAME>
    <CLIP_LEFT>0</CLIP_LEFT>
    <CLIP_RIGHT>933</CLIP_RIGHT>
    <SOURCE_TYPE>GENOMIC</SOURCE_TYPE>
    <SPECIES_CODE>XX</SPECIES_CODE>
    <SUBMISSION_TYPE>NEW</SUBMISSION_TYPE>
    <TEMPLATE_ID>PACH2306</TEMPLATE_ID>
    <TI>451443045</TI>
    <TRACE_DIRECTION>REVERSE</TRACE_DIRECTION>
    <TRACE_FORMAT>SCF</TRACE_FORMAT>
    <TRACE_NAME>PACH2306.y1</TRACE_NAME>
    <TRACE_TYPE_CODE>WGS</TRACE_TYPE_CODE>
    </trace>
    <trace>
    <CENTER_NAME>JGI</CENTER_NAME>
    <CLIP_LEFT>0</CLIP_LEFT>
    <CLIP_RIGHT>888</CLIP_RIGHT>
    <SOURCE_TYPE>GENOMIC</SOURCE_TYPE>
    <SPECIES_CODE>XX</SPECIES_CODE>
    <SUBMISSION_TYPE>NEW</SUBMISSION_TYPE>
    <TEMPLATE_ID>ACBG121468</TEMPLATE_ID>
    <TI>452858124</TI>
    <TRACE_DIRECTION>FORWARD</TRACE_DIRECTION>
    <TRACE_FORMAT>SCF</TRACE_FORMAT>
    <TRACE_NAME>ACBG121468.b2</TRACE_NAME>
    <TRACE_TYPE_CODE>WGS</TRACE_TYPE_CODE>
    </trace>
    <trace>
    <CENTER_NAME>JGI</CENTER_NAME>
    <CLIP_LEFT>0</CLIP_LEFT>
    <CLIP_RIGHT>872</CLIP_RIGHT>
    <SOURCE_TYPE>GENOMIC</SOURCE_TYPE>
    <SPECIES_CODE>XX</SPECIES_CODE>
    <SUBMISSION_TYPE>NEW</SUBMISSION_TYPE>
    <TEMPLATE_ID>ACBG121468</TEMPLATE_ID>
    <TI>452859852</TI>
    <TRACE_DIRECTION>REVERSE</TRACE_DIRECTION>
    <TRACE_FORMAT>SCF</TRACE_FORMAT>
    <TRACE_NAME>ACBG121468.g2</TRACE_NAME>
    <TRACE_TYPE_CODE>WGS</TRACE_TYPE_CODE>
    </trace>



    i need to extract this info in some way, and pass to celera assembler??

    or i need a special flag so celera assembler use this??

    thx in advance

    Cristian

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