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  • muzz56
    Member
    • Sep 2010
    • 14

    #1

    Maq error:[seq_read_fastq] Inconsistent sequence name

    Hi,
    I've been dowloaded some Illumina/Solexa short read files from SRA such as this one:

    @CCFFFFEFHHGHJJJJJGIIEHGIIJJGIIEGIEBGIJEEFFFFCFFFCDEDDDDDDDDDDDDDDDDBA@CDEEDCDCDDDDCCDCDBDDDDDDCCCDC
    @SRR350739.3 SOLEXA4_0073:2:1101:1220:2247 length=100
    CGAGATATTCAAGATTTCTCTGCTGCTTGTCAGTTGAGAGCGTTGCGATTGGATTCCCGTTCCTCCCCTTGGCTTCGGCTCAGTTCGTCCTTTAACATCA
    +SRR350739.3 SOLEXA4_0073:2:1101:1220:2247 length=100
    CCCFFFFFHHHDHHHIFIJIJIJIIIIJGIJIIJJIEIIJJJJIIIJEHGEHHIGIGHJIIIIIJHHHGFFFFFEEDDBDB>CCDCDDBDDDD>@CCC>C
    @SRR350739.4 SOLEXA4_0073:2:1101:1320:2116 length=100
    CACAAGGTCCCGGAACACACCACGCGTTGCGGCAGCGGCCATGAACACGGACAAAATACTGCGAGCCAATGCTGGGACCAAATCGCTAATCGTTCGCGAA
    +SRR350739.4 SOLEXA4_0073:2:1101:1320:2116 length=100
    CCCFFFFFHHHHHJJJJJJJJIJJJJJJIJJJJIFHHFFDDDDDDDDDDDBDDDDDDDDDEDDBDDDDDDDDDDDDDDDBBDDDDDDDDDDDDDDDDDDD

    and while trying to use the file in maq with the easy run command:

    maq.pl easyrun -d outdir ref.fasta reads.fastq

    I get this error :

    [seq_read_fastq] Inconsistent sequence name: CA>ACDADDDDCCB(<AC@@BBD-<99<?CD. Continue anyway.
    [seq_read_fastq] Inconsistent sequence name: BDBDBDDCDDA9A?<BCCCC>B. Continue anyway.
    [seq_read_fastq] Inconsistent sequence name: 5ACDDDDDCDDDDD. Continue anyway.
    [seq_read_fastq] Inconsistent sequence name: BDCABDDDDDDDDDBDCCCBDDBDBA<. Continue anyway.
    [seq_read_fastq] Inconsistent sequence name: CDC. Continue anyway

    A quick search around reveals that probably this error is due to spaces in the sequence name. Normally I'd remove the spaces in vim editor but these are huge files which can't even open in vim editor.

    Does anyone know an easy way to remove spaces in the sequence name or if I have a different problem other than spaces in sequence name?

    Thanks in advance.
  • maubp
    Peter (Biopython etc)
    • Jul 2009
    • 1544

    #2
    It looks like a different problem - those are not sequence names it is showing, but bits of quality strings.

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