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  • polsum
    Member
    • May 2009
    • 32

    #1

    Multiple Alignment of miRNAs with Precursors

    Hi I am looking for a program that can align all miRNA variants or isomirs to the precursers and produce a map like below. Can you please suggest a program for that? thanks in advance.

    PHP Code:
    >mmu-let-7f-2
    total read count                        2702595
    mmu
    -let-7f-5p read count                2702589
    remaining read count                    6
    exp                                     fffffff5555555555555555555555fffffffffffffffffffffffffffffff
    pri_seq                                 ugugggaugagguaguagauuguauaguuuuagggucauaccccaucuuggagauaacua
    pri_struct                              
    ...((((((.((((...((((.((......)).)))).)))).))))))...........    #MM
    a2b_26359701_x1                         ....gUaugagguaguagauuguaua..................................    1
    a2b_27273292_x1                         
    .....Uaugagguaguagauuguau...................................    1
    a2b_24052491_x48                        
    .....gUugagguaguagauuguaua..................................    1
    a2b_26138224_x2                         
    .....Aaugagguaguagauuguauagu................................    1
    a2b_27325497_x1                         
    .....Uaugagguaguagauuguauagu................................    1
    a2b_26941374_x1                         
    .....Aaugagguaguagauuguauaguu...............................    1 

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