How to deal with the RNA-seq reads in the overlpping regions of genome?
Hi,all
Now I'm dealing with the RNA-seq data from overlapping regions in genome(two genes from the forward and reverse strand are overlapping). As far as I know, the non-strand-specific RNA-seq data don't contain the strand information. So for the reads from the overlapping region, it's hard to distinguish their origin strand,especial for long overlapping regions . My work is to distribute the reads from the overlapping region.
Does anyone have done something similar with the question? As far as I know, most of the RNA-seq data are non-strand-specific. I want to know whether the work is meaningful or not, or there have existed ways to deal with the question. And I have come up with one way to solve the question. I just doubt the mean of the work.
Thanks!
Now I'm dealing with the RNA-seq data from overlapping regions in genome(two genes from the forward and reverse strand are overlapping). As far as I know, the non-strand-specific RNA-seq data don't contain the strand information. So for the reads from the overlapping region, it's hard to distinguish their origin strand,especial for long overlapping regions . My work is to distribute the reads from the overlapping region.
Does anyone have done something similar with the question? As far as I know, most of the RNA-seq data are non-strand-specific. I want to know whether the work is meaningful or not, or there have existed ways to deal with the question. And I have come up with one way to solve the question. I just doubt the mean of the work.
Thanks!