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I'm trying to compile bowtie for Windows x64 using MinGW64. I actually have a working binary for bowtie x64, but I can only get it to compile with BOWTIE_PTHREADS=0. When that is enabled, I get "Error: incorrect register '%eax' used with 'q' suffix" and make for bowtie fails. Anyone know any solution around this? I'm using pthreads from http://sourceforge.net/apps/trac/min...ile%20pthreads
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I have an operational (with threads) bowtie for Win7-x64 which I could make available if you wish. It comes with the usual disclaimer of warranty. I've run it for about a year with no problems. I will have to locate the notes for compiling though. -
That'd be great if you could provide me with it. If you have compiling notes to share too, that'd be helpful too for future/similar compilations. You wouldn't happen to have a working version of TopHat too?Originally posted by DWW1505 View PostComment
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I will to contact the researcher for whom it was built tonight. Please PM with your contact info as the files will be a bit much for email. TopHat (1.4.1 and 1.3.3), as implemented in my port, requires 64-bit Samtools, 64-bit Bowtie, several supporting files, and does not do .gz compression.Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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