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  • Oliviervg
    Member
    • Sep 2011
    • 13

    #1

    Fastx Trimmer : invalid sequence

    Hi everybody,

    I clipped my data (PE 2*76bp Illumina) with

    fastx_clipper -i s_1.fastq -o s_3_m50.fastq -l 50 -Q 33 -v
    and it seems to work.

    Then I want to trim my data with
    fastx_trimmer -i s_1_m50.fastq -o s_1_m50_trim10_500.fastq -f 11 -l 500 -m 50 -Q 33 -v

    and I get : fastx_trimmer: Error: invalid quality score data on line 124756 (quality_tok = "CCCFFFFFHGHHHJJJJJJJJII"

    I'm using : FASTX Toolkit 0.0.13

    Does anyone have an idea why I got an error ?
    Thank you

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