Ref Genes to Custom Genome

[puggie]

Hello,

Can anyone explain to me how I add a reference gene track to a custom genome? E.g. when viewing alignments i IGV I cannot see reference genes when choosing my own imported genome? The custom genome is mm9 + one additional non-murine chromosome.

tia

[Jim Robinson]

Hi tia,

If your chromosome names are of the form "chr1, chr2, chr3, ..." etc, you can download the following file from UCSC and just load it using "File > Load..." after selecting you custom genome.

http://hgdownload.cse.ucsc.edu/goldenPath/mm9/database/refGene.txt.gz

You can rename the file, but its important to keep "refGene" in the name, its how IGV knows it is this specific UCSC table format.

Jim