I've seen a few older threads on this, but no real consensus yet. Anyone have a script or method for finding circular DNAs (*osmids, minicircles) in an assembly? I suppose it's really just as "simple" as discovering the motif which is repeated and the beginning and end of the contig where it's circularized. Anyone attempted anything like this yet?
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I looked for plasmids in my assemblies by looking for contigs with the same sequence at both ends. I was able to verify that some of the contigs were plasmids by PCR and sequencing but I was not able to get a product for most of them though I was not very exhaustive in the primers that I tried. While this method works, I don't know that I would trust that you found circular DNA without a second verification.
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM
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