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  • kasutubh
    Member
    • Mar 2010
    • 25

    #1

    cuffdiff output

    Hi everyone,

    I want to analyze data using cummeRbund but I'm having problem with cuffdiff output. I don't get any entries in the gene_id and gene columns in the output files of cuffdiff. Also all the other files except isoform_exp.diff, isoform.FPKM_tracking are blank. When I specify file into the cummeRbund, I get following output,
    CuffSet instance with:
    2 samples
    0 genes
    0 isoforms
    0 TSS
    0 CDS
    0 promoters
    0 splicing
    0 relCDS
    What might be the problem here? I would really appreciate any help in this regard.
    Thanks in advance!
  • jp.
    Senior Member
    • Jul 2013
    • 142

    #2
    did you get solution? I got same and psoted here :
    Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc


    may expect your view ?
    Thank you


    Originally posted by kasutubh View Post
    Hi everyone,

    I want to analyze data using cummeRbund but I'm having problem with cuffdiff output. I don't get any entries in the gene_id and gene columns in the output files of cuffdiff. Also all the other files except isoform_exp.diff, isoform.FPKM_tracking are blank. When I specify file into the cummeRbund, I get following output,
    CuffSet instance with:
    2 samples
    0 genes
    0 isoforms
    0 TSS
    0 CDS
    0 promoters
    0 splicing
    0 relCDS
    What might be the problem here? I would really appreciate any help in this regard.
    Thanks in advance!

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