Unconfigured Ad

**chadn737** · 03-21-2012, 08:35 AM

Yes, use the samtools sort to create a sorted bam file. This is then appropriate as input into mpileup.

**Phantom123** · 03-21-2012, 09:12 AM

Originally posted by Phantom123 View Post

Do i have to do any sorting on the BAM file that Tophat generates before i convert it to a bcf/vcf file (using Samtools)?

Thanks for your help.

Thank you.

Topics	Statistics	Last Post
Genome Sequencing Reveals Large, Repeat-Rich DNA in Trapdoor Spider Named for Stephen Colbert by SEQadmin2 Started by SEQadmin2, 10-28-2026, 09:57 AM	0 responses 22 views 0 reactions	Last Post by SEQadmin2 10-28-2026, 09:57 AM
Single-Cell Sequencing of 5 Million Immune Cells Links Genetic Variants to Disease by SEQadmin2 Started by SEQadmin2, 10-28-2026, 09:51 AM	0 responses 14 views 0 reactions	Last Post by SEQadmin2 10-28-2026, 09:51 AM
Exeter-Led Study Maps What an Average Human Genome Looks Like by SEQadmin2 Started by SEQadmin2, 10-22-2026, 11:13 AM	0 responses 67 views 0 reactions	Last Post by SEQadmin2 10-22-2026, 11:13 AM
Long-Read Sequencing Uncovers Cancer Mutations Conventional Testing Missed by SEQadmin2 Started by SEQadmin2, 10-22-2026, 10:48 AM	0 responses 47 views 0 reactions	Last Post by SEQadmin2 10-22-2026, 10:48 AM

Tophat Bam file question