Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • hulan0@gmail.com
    Junior Member
    • Nov 2010
    • 2

    tophat2 segment_juncs error: Error: segment-based junction search failed with err =-6

    Hi,

    I am trying out the latest tophat2 on a small read file. My reference is the rabbit genome which has 3243 sequences (chromosomes + scaffolds).

    segment_juncs.log has the following lines at the end:

    >> Performing segment-search:
    Loading left segment hits...
    Error: invalid 'B' array subtype (^)!

    Has anyone had the same issue? How to fix it? Thanks a lot.

    L.
  • DineshCyanam
    Compendia Bio
    • Oct 2010
    • 35

    #2
    Re-download the tophat-2.0.0 package as it was updated and try again... (Same Version. Same file name)

    Originally posted by gpertea View Post
    There are quite a few different problems reported in this thread - I apologize for them. We are aware of a couple of these problems and we are trying to fix them, though we are encountering some difficulties reproducing some of these errors.
    We just updated the packages on the website with a couple of last minute fixes so please re-download the tophat-2.0.0 package of your choice (yes, it's the same version, same file names) from http://tophat.cbcb.umd.edu and try running the new version again on your test data. If tophat 2 still fails at any stage in the process please report the failure at [email protected] - it would also help if you attach a tarball or zip file with the content of the "logs" directory when you post a bug report there.

    Thank you,
    -Geo

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by SEQadmin2


      I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.


      Here are nine questions we think about, in roughly the order they matter, before...
      Yesterday, 07:11 AM
    • SEQadmin2
      From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
      by SEQadmin2


      Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


      The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
      ...
      06-02-2026, 10:05 AM
    • SEQadmin2
      Single-Cell Sequencing at an Inflection Point: Early Impacts of New Platforms and Emerging Trends
      by SEQadmin2


      With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.


      Introduction

      Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...
      05-22-2026, 06:42 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 06-17-2026, 06:09 AM
    0 responses
    20 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-09-2026, 11:58 AM
    0 responses
    38 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-05-2026, 10:09 AM
    0 responses
    44 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-04-2026, 08:59 AM
    0 responses
    49 views
    0 reactions
    Last Post SEQadmin2  
    Working...