Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts

  • gaffa
    replied
    I think I have encountered this problem - if I remember correctly I think the problem is that SMALT puts "unknown" as the value for the SO tag and that this value is not allowed by Picard. I think it worked fine after I changed these tags.

    Leave a comment:


  • bosTau2
    replied
    Are you trying to sort as well? Without sorting, picard worked for my Smalt bams.
    picard => samtools sort =>

    Leave a comment:


  • oiiio
    started a topic Picard with SMALT?

    Picard with SMALT?

    Has anyone ever tried to use Picard tools with SMALT? Specifically AddOrReplaceReadGroups and MarkDuplicates. Here is the error I get when trying to add read groups:

    Code:
    [Thu Apr 19 16:26:33 EDT 2012] Executing as gareth@farin on Linux 3.0.0-17-generic amd64; OpenJDK 64-Bit Server VM 1.6.0_23-b23; Picard version: 1.65(1160)
    INFO	2012-04-19 16:26:33	AddOrReplaceReadGroups	Created read group ID=foo PL=illumina LB=bar SM=GARETH
    
    [Thu Apr 19 16:26:33 EDT 2012] net.sf.picard.sam.AddOrReplaceReadGroups done. Elapsed time: 0.00 minutes.
    Runtime.totalMemory()=1901723648
    FAQ:  http://sourceforge.net/apps/mediawiki/picard/index.php?title=Main_Page
    Exception in thread "main" java.lang.IllegalArgumentException: No enum const class net.sf.samtools.SAMFileHeader$SortOrder.unknown
    	at java.lang.Enum.valueOf(Enum.java:214)
    	at net.sf.samtools.SAMFileHeader$SortOrder.valueOf(SAMFileHeader.java:59)
    	at net.sf.samtools.SAMFileHeader.getSortOrder(SAMFileHeader.java:240)
    	at net.sf.picard.sam.AddOrReplaceReadGroups.doWork(AddOrReplaceReadGroups.java:87)
    	at net.sf.picard.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:177)
    	at net.sf.picard.cmdline.CommandLineProgram.instanceMainWithExit(CommandLineProgram.java:119)
    	at net.sf.picard.sam.AddOrReplaceReadGroups.main(AddOrReplaceReadGroups.java:61)

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
23 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
20 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
36 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
42 views
0 reactions
Last Post SEQadmin2  
Working...