Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Bukowski
    Senior Member
    • Jan 2010
    • 388

    #1

    TopHat2 insertion bed outputs

    Unlike other people here I don't seem to have any problems actually running TopHat2 and the actually mapping results look pretty similar to the previous version of TopHat we were running.

    However it is reporting a lot more events in the insertions/deletions bed file. I will admit upfront that I'm using a home-built Bowtie index between the two rather than the iGenomes ones, but I don't understand why that would be responsible for this kind of output in the insertions.bed:


    1 567684 567684 A 13
    1 567684 567684 AT 10
    1 567684 567684 GTG 12
    1 567684 567684 AATC 2
    1 567684 567684 GATCG 8
    1 567684 567684 GATCGG 2
    1 567684 567684 CCTTACTACC 1
    1 567684 567684 CCTTACTACCAG 1
    1 567685 567685 T 1
    1 567685 567685 TG 12
    1 567685 567685 AGATG 1
    1 567685 567685 TATGATAGTGA 6
    1 567685 567685 TGAATATGATAGTGA 4
    1 567686 567686 G 2
    1 567686 567686 TC 2
    1 567686 567686 GAG 1
    1 567686 567686 TCAA 3
    1 567686 567686 GATGA 2
    1 567686 567686 ACCACACCTC 1
    1 567687 567687 CAGA 2
    1 567688 567688 A 3
    1 567688 567688 GGTGAAACC 1
    1 567688 567688 TAGATCGGAAGAG 1
    1 567689 567689 AAA 1
    1 567689 567689 CCCGC 1
    1 567689 567689 ATACTG 1
    1 567689 567689 GGAAGAG 3
    1 567689 567689 GGAAGAGCGT 1
    1 567690 567690 A 4
    1 567690 567690 TAG 1
    1 567690 567690 ATCAAACACA 1


    And this situations is repeated throughout the file to the tune of about 4 million entries.

    Any insight appreciated..

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Yesterday, 11:13 AM
0 responses
19 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, Yesterday, 10:48 AM
0 responses
18 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
36 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
42 views
0 reactions
Last Post SEQadmin2  
Working...