CDS annotation in CLC genomics?

evolgen

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Join Date: Apr 2012

Posts: 2
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CDS annotation in CLC genomics?

04-24-2012, 10:43 AM

I have de novo assembled a consensus ("reference") sequence for a non-model organism using RNAseq from Illumina and I want to annotate the contigs with CDS information. I have BLASTx'd everything against NCBI's protein data base (translating everything frist, of course) and now I would like to annotate the consensus contigs so that I can do a proper SNP analysis (i.e. I want to know which SNPs are synonymous/not, but I can't do this unless CLC knows where the coding regions are - and presumably this information comes from the BLAST results, but how do I put these together??)

Does anyone know how to do this? Or am I missing something?
Tags: annotation, blast web cgi ncbi, clc genomics workbench, snp analysis
JackMetal

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Join Date: May 2012

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#2

01-11-2015, 08:19 PM

Have you addressed this problem yet? I am having the same issue.
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CDS annotation in CLC genomics?

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