-
since alignments are alignments you could align them separately and output as SAM files then use Samtools to merge and sort the alignments. -
aligning multiple fastq for the same sample
Hi everyone, I am trying to align fastq against reference with bowtie. The input data contains same sample run in multiple lanes, 2, 3 or 4, as is commonly the case when expected depth could not be reached by single lane.
What could be the best strategy to align these, align one by one, or merge fastq first. The context is ChIP-Seq.
Thanks for response.
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Leave a comment: