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  • Kennels
    Senior Member
    • Feb 2011
    • 149

    #1

    Tools to identify read pairs?

    Hi,

    After quality filtering reads 1 and 2 of paired end reads, the pairing information is lost.
    I've been using the findpairs.py utility from the SHRiMP package to re-identify paired reads, but it requires large memory for large files, requiring one to split the files up. It works fine, but can anyone suggest other such tools?

    Thanks
  • Kennels
    Senior Member
    • Feb 2011
    • 149

    #2
    I found this thread which implements a custom script and talks about others to do this job (with limitations):
    Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc


    If there are other tools please let me know, thanks!

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