about the tophat and cufflinks
Hello professors
I meet a problem during using the tophat and cufflinks to assembly our reads. We follow the instrunction of the protocol"Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks",but we get a abnormal results of that the CDS is zero while the genes isoforms TSS and promoters are all correct.what the problem?
I meet a problem during using the tophat and cufflinks to assembly our reads. We follow the instrunction of the protocol"Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks",but we get a abnormal results of that the CDS is zero while the genes isoforms TSS and promoters are all correct.what the problem?
