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  • lukas1848
    Member
    • Jun 2011
    • 54

    #1

    Fixing mate pairs in fastq

    Hi again,

    I have some raw fastq files directly from illumina and somehow the PE files are out of sync. I hence need a script to fix these files. So far I used a perl script by Konrad Paszkiewicz (fastq_pe_even.pl), but after CASAVA's read identifiers were updated, it doesn't work anymore.
    So far, I wasn't able to change the script successfully.

    (btw. I used trimmomatic to clean my raw reads and apparently it could handle the messed up fastq files. It didn't fix them in the end, though)

    Could someone provide an up-to-date script for simply fixing PE fastq files?
  • kga1978
    Senior Member
    • Nov 2010
    • 100

    #2
    Have a look at this

    http://seqanswers.com/forums/showthread.php?t=17974

    I have been using this:

    http://seqanswers.com/forums/attachm...8&d=1330350938

    Comment

    • lukas1848
      Member
      • Jun 2011
      • 54

      #3
      thanks, that's exactly what I need.

      Comment

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