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  • Wallysb01
    Senior Member
    • Feb 2011
    • 286

    #1

    problems with SRA Toolkit setup for csra

    I'm trying to set up the newest SRA toolkit to be able to deal with these new csra files, but I can't seem to get the configuration-assistant.perl script to work correctly. It appears I don't have the scheme files loaded or installed?

    I simply uncompressed the Mac 64 bit binaries and run the configuration-assistant script and get the following:

    Code:
    ==========================================
    The SRA toolkit documentation page is
    http://www.ncbi.nlm.nih.gov/Traces/sra/std
    ==========================================
    
    checking for vdb-config (local build)... no
    checking for vdb-config (./vdb-config: found)... yes
    checking configuration files... '/Users/Walter/.ncbi/ncbi-config.kfg
    /Users/Walter/Bioinformatics/Tools/sratoolkit.2.1.10-mac64/bin/ncbi/config.kfg
    /Users/Walter/Bioinformatics/Tools/sratoolkit.2.1.10-mac64/bin/ncbi/vdb-copy.kfg'
    checking refseq configuration... paths=/Users/Walter/ncbi/refseq
    checking /Users/Walter/ncbi/refseq... ok
    checking /Users/Walter/ncbi/refseq for invalid empty reference files... none found
    checking krypto configuration... pwfile=/Users/Walter/.ncbi/.vdbpass
    Do you want to update VDB password? [y/N] Y
    checking for vdb-passwd (local build)... no
    checking for vdb-passwd (./vdb-passwd: found)... yes
    Changing password
    New password:
    Retype new password:
    password updated ok
    checking schema configuration... not found
    --------------------------------------
    WARNING: SCHEMA FILES CANNOT BE FOUND.
    IT COULD CAUSE LOADERS TO FAIL.
    --------------------------------------
    Does anyone have a suggestion, this seems new and under documented.
  • srasdk
    Member
    • Jun 2011
    • 19

    #2
    This is a warning - it will not affect you since you will use dumpers (fastq-dump, etc...), not loaders

    Comment

    • xhyuo
      Junior Member
      • Jun 2012
      • 6

      #3
      Originally posted by srasdk View Post
      This is a warning - it will not affect you since you will use dumpers (fastq-dump, etc...), not loaders
      Hi, i have a question when i used SRA.

      First, it worked well in Windows system as i used fastq-dump on the .csra file to generate the .fastq file. But such things were not happened in the Linux system.
      And i want to use sam-dump. However, every time it said that "sam-dump has stopped working" in the windows system.

      How you ever met that problem before?

      Comment

      • srasdk
        Member
        • Jun 2011
        • 19

        #4
        No. "stop working" may mean the program is crushing. What SRR accession are you looking at?

        Did you try contact SRA help desk?
        go to SRA home page:

        and click "Write to help desk" in the lower right corner

        Comment

        • xhyuo
          Junior Member
          • Jun 2012
          • 6

          #5
          Originally posted by srasdk View Post
          No. "stop working" may mean the program is crushing. What SRR accession are you looking at?

          Did you try contact SRA help desk?
          go to SRA home page:

          and click "Write to help desk" in the lower right corner
          Thank you for your reply!

          Now it works. I've already sam-dump the .csra file and then transferred it into .bam file.

          One question occurred to me that since the raw data i got .csra file is documented as paired-end. It should be two files per sample. Am i right?
          Are there any problems in the .bam file i got?

          Thanks!

          Comment

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