Reads for a gene

[biochembug]

Hi Friends

I am interested to know whether an isoform of a protein (at transcript level) is expressed in different human tissues or cells. My plan is to dig the already available deep sequencing data available at NCBI using readily available tools.

Information about tools specifically meant to filter out reads from a fastq file for a given transcript sequence or a set of sequences will be really helpful?

Biochembug

[gaffa]

Maybe this could be of interest: http://www.plosone.org/article/info%...l.pone.0019816

[biochembug]

Seems interesting!

Thank you.