Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • ATD
    Junior Member
    • May 2012
    • 1

    Cufflinks 2.0.0 installation errors

    Hello,
    I have tried these steps http://seqanswers.com/forums/showthr...?t=4589&page=3.

    However, cufflinks 2 installation doesn't work. I tried all 3 verions of Boost 1.47 to 1.49.

    We are running RHEL5.4
    Linux 2.6.18-194.11.4.el5 #1 SMP Fri Sep 17 04:57:05 EDT 2010 x86_64 x86_64 x86_64 GNU/Linux

    The boost configure is

    ./bjam --prefix=$HOME/local --toolset=gcc architecture=x86 link=static runtime-link=static stage install

    The cufflinks configure is
    ./configure --prefix=$HOME/local --with-boost=$HOME/local --with-bam=$HOME/local --with-eigen=$HOME/local


    The error is
    libcufflinks.a(assemble.o):/home/atd/local/include/boost/thread/tss.hpp:84: more undefined references to `boost::detail::get_tss_data(void const*)' follow
    collect2: ld returned 1 exit status
    make[2]: *** [cufflinks] Error 1
    make[2]: Leaving directory `/home/atd/tmp/cufflinks-2.0.0/src'
    make[1]: *** [all-recursive] Error 1
    make[1]: Leaving directory `/home/atd/tmp/cufflinks-2.0.0'
    make: *** [all] Error 2


    Please help me.
    Thank you.
    Andy
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Is the boost library path correctly set? See this thread for some additional info: http://seqanswers.com/forums/showthread.php?t=19805

    Comment

    • Verbrugghen
      Junior Member
      • Jan 2020
      • 1

      #3
      This boost version has some changing and these changes are good for everyone we can now manage the different cases at one time. This version is too much helpful for the book report writing services so that’s why book writers buy this paid version for unlocking all the services.

      Comment

      • samnantha99
        Junior Member
        • Apr 2026
        • 1

        #4
        It looks like the issue is related to Boost thread libraries not being properly linked. Errors like undefined references to boost::detail::get_tss_data usually mean that -lboost_thread and -lboost_system are either missing or mismatched with your build (static vs shared).

        Since you're building Boost with static linking, make sure Cufflinks is also linking against the same static libraries and that your LD_LIBRARY_PATH or LIBRARY_PATH includes $HOME/local/lib. You might also want to explicitly pass the libraries during configure or edit the Makefile to include:
        -lboost_thread -lboost_system -lpthread

        Also double-check that the Boost version you compiled matches the compiler used for Cufflinks.

        If you're working on this as part of a bioinformatics assignment and need a clearer breakdown of the setup or troubleshooting steps, you can also get help here: https://essaypro.com/do-my-assignment

        Comment

        Latest Articles

        Collapse

        • SEQadmin2
          Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
          by SEQadmin2



          Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
          ...
          Yesterday, 11:10 AM
        • SEQadmin2
          Cancer Drug Resistance: The Lingering Barrier to Rising Survival
          by SEQadmin2



          Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

          There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
          07-08-2026, 05:17 AM
        • GATTACAT
          Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
          by GATTACAT
          Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
          07-01-2026, 11:43 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, Yesterday, 10:04 AM
        0 responses
        11 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 07-08-2026, 10:08 AM
        0 responses
        9 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 07-07-2026, 11:05 AM
        0 responses
        17 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 07-02-2026, 11:08 AM
        0 responses
        31 views
        0 reactions
        Last Post SEQadmin2  
        Working...