help - convert .bed to .bam

[adurasiwam3]

hi,

i need to convert a .bed file to .bam. I've tried using BEDTools, but i've been running into a lot of issues with it.

are there any other tools/methods out there to do this?

thanks

[arvid]

What are the issues with bedtools? If you post a few lines of your bed file, maybe these issues could be sorted out.

[adurasiwam3]

hi,

thanks for your reply.

so the current issue i'm having is:

Error: BED entry without name found at line: 1. Exiting!

the command I gave in UNIX is:

bedToBam -i chr1.bed -g hg18.txt > chr1.bam

chr1.bed (bed file for only chromosome1) looks like this:

chr1 100000000 100000036
chr1 100000040 100000076
chr1 100000090 100000126
chr1 100000136 100000172
chr1 100000189 100000225
chr1 100000235 100000271
chr1 100000287 100000323
chr1 100000336 100000372
chr1 100000401 100000437
chr1 100000452 100000488
chr1 100000512 100000548..
.....

and hg18.txt looks like this:

chr1 247249719
chr10 135374737
chr11 134452384
chr12 132349534
chr13 114142980
chr14 106368585
chr15 100338915

thanks!

[Hobbe]

The bed file seems very sparse to me. BedToBam seems to be looking for a name which should be in a fourth column. Haven't really tried BedToBam, but that is my guess.

[arvid]

Yes, that's the problem. Try the following:

cat chr1.bed | awk '{x++; printf "%s\tread%d\n",$0,x}' | bedtools bedtobam -g hg18.txt -i - > chr1.bam

I get the following output then, when converted to SAM (with samtools view):

read1   0       chr1    100000001       255     36M     *       0       0       *       *
read2   0       chr1    100000041       255     36M     *       0       0       *       *
read3   0       chr1    100000091       255     36M     *       0       0       *       *
read4   0       chr1    100000137       255     36M     *       0       0       *       *
read5   0       chr1    100000190       255     36M     *       0       0       *       *
read6   0       chr1    100000236       255     36M     *       0       0       *       *
read7   0       chr1    100000288       255     36M     *       0       0       *       *
read8   0       chr1    100000337       255     36M     *       0       0       *       *
read9   0       chr1    100000402       255     36M     *       0       0       *       *
read10  0       chr1    100000453       255     36M     *       0       0       *       *

[adurasiwam3]

thanks a lot for helping out! really appreciate it.