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  • crader
    Member
    • Sep 2010
    • 12

    #1

    Where do the cufflinks P-values from DE comparison between 2 individuals come from?

    Hi,

    I've used cufflinks for DE analysis among pooled samples of individuals (n=20) to build a transcriptome. There are 3 exposures that have their own run data, but comparison between 2 exposure concentrations using cufflinks has provided p-values.

    Refining further analysis by fold change and p-value provides a gene list that makes sense to the system- though we will only use fold change and commonly up/down regulated genes to provide candidates for further analysis.

    Where have these p-values come from? Should I just ignore them?

    Many thanks!

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